Friday, December 20, 2019

DNA Barcoding and the BOLD Database - 1187 Words

Many technological advancements have been made since the discovery of DNA structure and its function in 1953 by James Watson and Francis Crick. Recently, scientists have developed a process called DNA barcoding. The result of DNA barcoding is a database of barcodes of species, made up of a sequence of nucleotides in specific genes. DNA barcoding is a relatively new process that isolates a section of a mitochondrial gene and amplifies it, allowing scientists to sequence the order of nucleotide bases in a certain gene, the organism’s â€Å"barcode.† The mitochondrial cytochrome c oxidase 1 gene (CO1) is used for animals. It codes for a protein that is a part of the electron transport chain, which is used by every species during oxidative phosphorylation in cellular respiration. Every organism has a unique CO1 gene, but organisms that are within the same families will have more similar genes. Thus, by looking at a sequence of nucleotides in the CO1 gene, scientists can ide ntify and categorize species. Each DNA barcode is registered in the Barcode of Life Database (BOLD) under its respective species, and barcodes are being continually added to this database by scientists on the grassroots level. This allows easy and fast identification of species with no expert knowledge of the species required. The old Linnaean system of taxonomy is now outdated, replaced by this revolutionary new way to identify organisms. Today, scientists are utilizing the DNA barcoding process to answer aShow MoreRelatedDNA Barcoding Project1519 Words   |  6 PagesDNA Barcoding Project – Individual DNA Sequence Analysis Worksheet Please answer the following questions for each sample that you had sequenced: (DO NOT USE TRACK CHANGES) Sample (Sequence) Name: 08-03-MJ-A_COI_FOR.ab1 1. At what base pair (bp) does the good quality sequence start (e.g. G35)? _____C46______ Do not crop the sequence until Step 4. Highlight the first base, take a screen shot of the waveform (should be full length, not just a few bases) and paste in a screen shot of thisRead MoreDna Barcoding Using Coi For Species Identification For Conservation1573 Words   |  7 PagesDNA Barcoding using COI for Species Identification for Conservation Introduction and Literature Review The DNA Barcoding is becoming more popular in the present times due to its accuracy in the identification of different species. It has been approved to be more accurate than other taxonomic methods. The DNA Barcoding employs in the use of Polymerase Chain Reaction to magnify the COI gene. The amplified COI genes of the organisms are sequenced and compared with a known database of the organismsRead MoreDna Barcoding And Its Effects On The Malaysian Market1964 Words   |  8 Pagesutilize the DNA barcoding in the fish segment, to assess the frequency of fish mislabeling on the Malaysian market (2015). DNA barcoding tries to standardize scientific classification by using short, single DNA sequence, for example, part of the mitochondrial gene COI to distinguish between species (Larson, 2007). The process begins with extracting DNA and using primers to amplify COI regions from the DNA extracts via PCR. The sequences attained are compared to the available sequences in Bold and GenBank

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